- 技術簡介
- 實驗數據
- 發表文獻
藍景科信將探針雜交捕獲技術和NGS技術結合，提供被子植物353個單拷貝核基因靶向捕獲測序服務。為研究被子植物的系統進化和分類、植物適應性機制、種群進化歷史、種群的結構、基因滲透和漂移提供高效的整體解決方案。靶向捕獲測序技術服務，使用生物素化的探針，捕獲被子植物353個單拷貝核基因。您可以提供新鮮植物樣本，臘葉標本或者古老的博物館標本，我們負責DNA提取、文庫構建、靶向捕獲、測序和數據分析。
技術流程
Fig 1. Heatmap of Gene Recovery Efficiency. Each row is one sample, and each column is one gene. Colors indicate the percentage of the target length (calculated by the mean length of all k-medoid transcripts for each gene) recovered.
Fig 2. Total Length of Sequence Recovery for Both Coding and Non-coding Regions Across 353 Loci for 42 Angiosperm Species. Reads were mapped back to either coding sequence (yellow) or coding sequence plus flanking non-coding (i.e. intron) sequence (purple)… The total length of coding sequence targeted was 260,802 bp. The median recovery of coding sequence was 137,046 bp and the median amount of non-coding sequence recovered was 216,816 bp (with at least 8x depth of coverage).
參考文獻：
Johnson MG, Pokorny L, Dodsworth S, Botigué LR, Cowan RS, Devault A, Eiserhardt WL, Epitawalage N, Forest F, Kim JT, Leebens-Mack JH, Leitch IJ, Maurin O, Soltis DE, Soltis PS, Wong GK, Baker WJ, Wickett NJ. A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-Medoids Clustering. Syst Biol. 2019. 68(4):594-606. doi: 10.1093/sysbio/syy086.
使用被子植物353個單拷貝核基因捕獲探針進行靶向捕獲測序，發表的部分文獻：
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